FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_dakar-41667_rr.351000000a9b28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_dakar-41667_rr.351000000a9b28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2583589
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGGTTTTGGCGATACTAGCCTTCTTGAGATTCACAGCAATCAAGCCA61810.23924083900341733No Hit
ATTCCATACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTTCCAT49900.19314217547760112No Hit
CTACATGTACTTGGACAGGAGCGATGCTGGTAAGGCCATTTCTTTTGCTA37300.14437280852333712No Hit
CTCTATTCCATACCATGAGCATGTCCTCAGTAGTCATCCATTCTCCCTTT34180.1322965843251384No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA30610.11847859702143027No Hit
CATCAATAGATGGGGTTCCGTGGGGAAGAAGGAGGCTATGGAAATAATAA30320.11735612746454642No Hit
GGCTTTTTCCGTGCCATGGCGTTATCGGCCTGACTATGAGCTTGGTTTCC29620.11464671818930952No Hit
TATTTACACAGTGACAAGAAATGCCGGTCTGGTTAAGAGACGTGGAGGTG29590.11453060064894223No Hit
CTATAAGGGATCCACACCATAAGTCCTCCCTTTTCCCCAAATAGGGAATG28620.11077613351039967No Hit
CCATAAGTCCTCCCTTTTCCCCAAATAGGGAATGTCTGTCCATTTTGTTA27830.1077183716140609No Hit
GTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAA26580.10288014076542361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCCC100.0070915883144.99944145
CGGCGCC203.8668164E-4108.74958145
GCGACAG900.0104.736011
GCGTCAG259.3835365E-487.011451
TTCGAGT3850.086.624722
CGACAGT1100.085.683142
GTTCGAG3900.085.524071
CGAGTTT3900.085.512494
ACCGTTT451.1942375E-464.4442145
TTCCGCC350.003567197662.142616145
ACAGTTC1550.060.8062174
CAGTTCG1550.060.8062175
AGTTCGA1600.058.9060256
TGCACCC502.0108817E-457.999775145
CGTCAGA400.006051594454.3758432
GCTTTTT8700.053.3341562
GAAGCGA7000.052.8212246
TGAAGCG7000.052.8212245
AAGCGAG7000.052.8212247
AAATCTC553.22002E-452.727075
Produced by FastQC (version 0.11.2)