FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_472_rr.351000000a9a0f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_472_rr.351000000a9a0f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802641
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT71160.8865732002227646No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC29390.3661661938525443No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT23680.2950260452680588No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT21400.26661982131488426No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC13940.1736766499593218No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA13710.17081110982369452No Hit
GTATGCAGAAACCAACCTGCTGCAACGGCACTGAATACAACAATAACCTC13160.1639587312384989No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT12680.15797847356414638No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT12480.15548669953316613No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA11310.1409098214519318No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT11030.1374213378085595No Hit
GAACAAAATAGTACCAAATTGAGTACTATTTCCTTTTATTCATCCATTAT10930.13617545079306936No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT10770.13418203156828518No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC10650.13268696714969708No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAAT10020.12483787895210935No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA9930.12371658063816825No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAACCTGAGACCAC9500.11835926647156077No Hit
GGATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAGGCCAAATG9480.11811008906846274No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT9220.11487078282818844No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC8580.10689710592905172No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT8490.10577580761511063No Hit
CTGTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTC8390.1045299205996205No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT8260.10291026747948336No Hit
GTAGAAACACGAGCATTTTTGATTCTTGTTTCTTTTTATTGGATGTTTAG8210.1022873239717383No Hit
ATTCAATATCTGCGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA8160.10166438046399325No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC8090.10079225955315016No Hit
CCATTCATTGAACCAGTATACACTCTGTATCACCCAAGGGTTGTTGCTAA8090.10079225955315016No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC8060.10041849344850312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC253.7083737E-8145.01561
GCCCATA100.007088327145.015564
GTAACAA11650.0124.476884
GAGCAGA1000.0123.263251
GAAACAC5250.0116.012474
CGGTGCA2350.0114.1611942
AAACACG5650.0107.799195
TAGAAAC5900.0105.689322
ACGTGCA750.096.6770552
TAGTAAC15200.096.359032
TAACAAG15300.094.7814255
GGAGCAT700.093.224293
CAGCCTT1650.092.282645
CACGCAC406.112459E-790.634733
CGGAGCA750.087.0093462
AGCGTTT353.4350323E-582.8660356
TCTACTA353.4350323E-582.8660355
GCGGAGC801.8189894E-1281.571261
GAGCATC6150.081.3502044
AACACGA7150.081.1275946
Produced by FastQC (version 0.11.2)