FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_468_rr.351000000a99f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_468_rr.351000000a99f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068031
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT61080.5718935124542265No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC31570.2955906710573008No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT27710.25944939800436506No Hit
GGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGAATTGCAACCAG24440.22883230917454642No Hit
TAATATATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACC23760.22246545278180127No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA19830.18566876804137708No Hit
CAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTT19590.18342164225570232No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA18330.17162423188090983No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC17660.16535100572923445No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT16890.15814147716686128No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT14670.1373555636493697No Hit
GCCAAAAACACAATGGCAGAATTTAGTGAAGATCCTGAATTGCAACCAGC14270.13361035400657845No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC13960.13070781653341523No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT13730.12855432098881026No Hit
GGATACAACTTAAAACAAATGCTGAAGACATAGGAACCAAAGGCCAAATG12910.12087664122108815No Hit
GGTGGATTAATACCTTTTTGCCAGGATATCATTGATTCATTAGACCGACC12330.11544608723904082No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT12110.11338622193550563No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT11990.11226265904266824No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT11750.11001553325699347No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA11620.10879834012308633No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA11580.1084238191588072No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG11550.10814292843559785No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT11350.1062703236142022No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA11210.10495950023922526No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT10740.10055887890894552No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCGGCTGG10720.10037161842680596No Hit
AACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTATTCAAT10690.10009072770359662No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG100.0070872824145.024951
TTCGTCC100.0070912517144.9978145
CGCGATC100.0070912517144.99783
GTAACAA14450.0114.392734
CGGTGCA3700.0111.6874852
TTCGGAA203.8663638E-4108.74835145
GAAACAC5950.0101.132934
AAACACG6250.096.2785345
TAGAAAC7150.090.243382
GAGCAGA2250.090.2377551
GAGCATC6100.089.137994
TAGTAAC19100.087.3023452
TAGTAGA1600.086.0924453
AAGCGGA353.4376433E-582.855882
AGCATCT7300.081.437125
TAACAAG20350.080.871015
ACAAGAG20750.079.312057
AACACGA7650.072.972756
AGCACGC502.3039902E-672.512481
AGCGTTT406.663897E-572.49895
Produced by FastQC (version 0.11.2)