FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_463_rr.351000000a99e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_463_rr.351000000a99e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463094
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT21900.4729061486436879No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC13230.28568713911214566No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT10840.23407774663459255No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT10530.2273836413341568No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC8650.18678713177022374No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT6350.13712118921860358No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC6310.13625743369596668No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT6200.1338821060087153No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC6180.13345022824739686No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT6060.13085896167948624No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTTCCTACATGCT5800.12524455078234656No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT5420.11703887331729627No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT5120.11056070689751973No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG5040.10883319585224599No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA5030.10861725697158676No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT4890.1055941126423577No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC4760.10278690719378787No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC4670.1008434572678549No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGGA202.1282885E-6145.015011
ATACGCT100.007087447145.015013
TATACGC100.007087447145.015012
AACACGT151.2290753E-4145.015016
AGCACGC351.899025E-9124.2985841
AGCGGAG256.4578544E-6116.012012
GTAACAA6800.0110.893844
CACGCAC404.80577E-9108.761263
CGCACTT404.80577E-9108.761265
ACACGTG203.8648004E-4108.749527
CTACGCC203.8648004E-4108.74952145
CGGTGCA1650.0105.465462
GCACGCA451.08957465E-896.6766742
GCGGAGC750.096.676671
TAGTAAC8000.094.259762
GAAACAC2850.094.1325454
CAGCATC700.093.223944
AAACACG2900.092.5095755
TAGAAAC3150.092.073022
GAGCATC2500.089.909314
Produced by FastQC (version 0.11.2)