FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_460_rr.351000000a99d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_460_rr.351000000a99d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832464
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT21720.26091218358992097No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA17740.2131023083280478No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT16330.19616463895135405No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG15830.19015837321493784No Hit
GTATATGCATACAATAGGATATTATTCATCCATAAAAGGGAATGAAGTAT15410.1851131099963482No Hit
GTCTATTGGAATTGAAGGGTTTGAGCCATACTGTATGAACAGTTCCTCAA15080.18114897461031348No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC14860.17850621768629035No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA14730.17694458859482212No Hit
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA13740.165052182436718No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC12320.14799438774529589No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT12010.14427050298871782No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG11900.14294912452670627No Hit
CTTTATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAG11830.142108247323608No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT11810.14186799669415134No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG11710.1406667435468681No Hit
ATATAAAGAGATCCATATCTAGACACATTTAAATCAAAGTGTTGAAATAC11610.13946549039958483No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG11390.1368227334755617No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT11250.13514097906936517No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA10730.12889446270349228No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG10730.12889446270349228No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT10430.12529070326164254No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG10320.12396932479963098No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG9980.11988506409886794No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC9930.1192844375252263No Hit
GAGCCATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAA9780.11748255780430145No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT9650.11592092871283322No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA9320.11195679332679852No Hit
CTTTATATTACTTGAAGTTTACTGGGCTTCTTGGGTGCATAATGTTTTAA8890.10679140479348055No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA8870.1065511541640239No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA8830.10607065290511061No Hit
CCCTAAGGAAGAGGAAGAATATGATTTTCAGACTTGTCATGTTATGTTAC8690.10438889849891406No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG8660.10402852255472908No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT8630.10366814661054412No Hit
GTCTAATATCTTCTTTAATTTTGAAGAATAGCTTTACTGGTGCAGAATTC8430.10126564031597762No Hit
CCTATGATACATATGTTGCATCACACGTATGAGGCTCTATTCATTTTTAT8400.10090526437179265No Hit
ACACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGA8380.100665013742336No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAC203.8640373E-4108.7626651
GCACGCA800.0108.756132
CGCACTT900.096.672115
TATGGGC259.38409E-487.0049064
GTAACAA12800.079.301344
AGCACGC1100.079.100111
AAACACG3050.078.447045
CACGCAC1200.072.504093
AACACGA3150.071.353236
TAGTAAC14500.070.5039752
CGGTGCA1150.069.351732
ACACGAG3250.069.1577457
GAGCATC1950.066.926844
CGGACCA1750.066.289455
GAAACAC3550.065.355794
TAACAAG15600.065.0677645
TTACCGG1850.062.711
ACCGGAC1850.062.7062343
TACCGGA1850.062.7062342
AGTAACA16550.062.2089423
Produced by FastQC (version 0.11.2)