FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_451_rr.351000000a99ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_451_rr.351000000a99ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814464
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT30210.37091878830740216No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA22940.28165763004871913No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG22810.2800614882916863No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT21360.26225836869401226No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA18320.22493320760647492No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC18310.22481042747131857No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC17780.21830308030803078No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG15170.18625746503221752No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG14490.17790841584158418No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC14210.1744705720572057No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT14150.17373389124626748No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA13670.1678404447587616No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG13250.16268367908219394No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT13090.16071919691969197No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT12650.15531687097281158No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG12350.15163346691812038No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA12070.14819562313374196No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC11610.1425477369165488No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA11510.14131993556498507No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC11420.14021491434857772No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA11250.13812765205091937No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC10800.1326025459688826No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG10750.13198864529310073No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG10690.1312519644821625No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT10140.124499057048562No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG9970.12241179475090365No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT9820.12057009272355808No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT9460.11615000785792864No Hit
CTGCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAA9120.11197548326261197No Hit
GATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG8910.10939710042432815No Hit
AAATACAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGA8550.10497701555869873No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG8420.10338087380166588No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG8330.10227585258525854No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACA8280.10166195190947666No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC8270.10153917177432029No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT3300.0114.2420355
GCACGCA3650.0111.23252
AGCACGC3700.0109.736111
CACGCAC4000.0101.499663
CGGTGCA2600.089.230462
GTAACAA13250.087.5468754
AGTGGTC259.386243E-486.99971145
TAGTAAC13700.084.671252
ACGCACT5000.082.649724
TAACAAG16800.069.478935
AAACACG4100.067.194895
AGTAACA17900.064.804253
ACAAGAG22150.052.370027
GTAGTAA23100.052.102591
AACACGA4750.050.368256
AGCATCT6650.049.0599825
AGCGGAG901.3233002E-648.333172
TCGGTTG450.00963862748.33317145
GTAGAAA9100.047.804971
GAAACAC6150.047.1543124
Produced by FastQC (version 0.11.2)