FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_450_rr.351000000a99bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_450_rr.351000000a99bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788874
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT44580.5651092569916109No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA34210.4336560718188202No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG34110.43238844226074125No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA27570.3494854691623757No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC26200.3321189442166937No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG23850.30232964960183756No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT22560.2859772283026187No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC22510.28534341352357917No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA22380.2836954950980765No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG22220.28166728780515016No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT22140.28065318415868695No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG21610.2739347475008683No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG20440.2591034816713442No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT18820.23856788283046468No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA18690.23691996440496202No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG18650.23641291258173042No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA18250.2313423943494145No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG17760.22513100951482745No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT17600.2231028022219011No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC17540.2223422244870537No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC16770.2125814768898455No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC16720.21194766211080604No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT16620.21068003255272702No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA16080.20383483293910054No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGT16000.20282072929263734No Hit
ACACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGA15870.20117281086713465No Hit
CTGCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAA15600.1977502110603214No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG14320.18152455271691043No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC13770.174552590147476No Hit
AAATACAATTGGACCGATTACCCCTCAACACCAGAGAGGTGCCTTGATGA13750.17429906423586022No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG13100.16605947210834685No Hit
GTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCAATTCTTTTAC13020.16504536846188364No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT12920.16377773890380465No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG12660.1604819020527993No Hit
AGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGA12460.15794664293664135No Hit
GGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGTCAAGAATAAA12400.15718606520179396No Hit
GAGCCATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAA12270.15553814677629127No Hit
GATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG12150.1540169913065965No Hit
AATAGCAACTGTACGAAATACAATTGGACCGATTACCCCTCAACACCAGA11930.15122820627882272No Hit
TTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATCA11710.14843942125104898No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT11440.1450168214442357No Hit
GCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCCCGGTCAAGAC11370.14412948075358042No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT11290.14311537710711722No Hit
GTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATGATA11270.14286185119550143No Hit
CTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATGATAATGAT11260.14273508823969355No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAA11160.14146745868161453No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT10920.138425147742225No Hit
CTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCA10840.1374110440957618No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT10820.137157518184146No Hit
GTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACCAGTC10500.13310110359829325No Hit
GGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCAGAG10480.13284757768667746No Hit
ACATAGAGGAAGAACCAGAGGATGTTGATGGCCCAACTGAAATAGTATTA10450.1324672888192538No Hit
GTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAACAACATGACCACAA10390.13170671108440637No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG10380.1315799481285985No Hit
CATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTCA10300.13056584448213532No Hit
AGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGAC10130.12841087423340103No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG10100.12803058536597733No Hit
GTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTTAC9990.12663619285209043No Hit
ATTTCATACAATGTTTCTAATAATGATAATGATACAACCATATCAGCTAT9980.12650942989628255No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC9940.12600237807305095No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC9860.12498827442658778No Hit
ATTTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTATGA9850.12486151147077987No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT9810.12435445964754828No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT9800.12422769669174039No Hit
ATCTATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAAT9730.12334035600108509No Hit
GGATTTGTTTAGTCACTGGCAAACAGGAAAAATGGCGAACAACAACATGA9720.1232135930452772No Hit
ACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAG9710.1230868300894693No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAACAGACGGATTCATAA9680.12270654122204559No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGTTTGACA9660.1224530153104298No Hit
CCTATTTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTA9650.12232625235462191No Hit
CAATTGGACCGATTACCCCTCAACACCAGAGAGGTGCCTTGATGACATAG9560.12118538575235083No Hit
CCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGATGG9390.11903041550361654No Hit
GAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGAAGTAAACACTCAGA9340.11839660072457707No Hit
TGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGA9330.11826983776876915No Hit
ATTCTGGAGTGCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCC9250.11725573412230597No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT9230.11700220821069017No Hit
GTGGAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTT9190.11649515638745857No Hit
GTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGGTC9140.1158613416084191No Hit
GATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTTACCAA9100.1153542897851875No Hit
GCTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATC9030.1144669490945322No Hit
ACTCTGCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAA9010.11421342318291641No Hit
TTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTTAG9010.11421342318291641No Hit
GATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCC8890.11269226771322162No Hit
TATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTG8880.11256550475741373No Hit
GGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACCGCAGT8830.11193168997837424No Hit
TTATGAATCCGTCTGTTGGAATTGAAGGGTTTGAGCCATACTGTATGAAC8820.11180492702256634No Hit
ATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGC8790.11142463815514264No Hit
CTTCTGGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACC8690.11015700859706368No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC8590.10888937903898467No Hit
GTTTGAGGAATGTTCCATTTACCAACACTCTCAAGGACAATACATTACGC8550.1083823272157531No Hit
GTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACAC8440.1069879347018662No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCCTCTGGTTCTTCCTCTA8280.10495972740893983No Hit
GAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTCCATTT8190.10381886080666874No Hit
ACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAGTGCTATGAAAG8150.10331180898343714No Hit
GCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTG8080.10242446829278186No Hit
CCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATG7930.10052302395566338No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAAG100.0070866616145.026812
TATCGGG100.0070906924144.99924145
AGCACGC700.0113.949641
GAGCAGA2400.0111.7915041
CGGTGCA1300.0105.981132
CACGCAC800.099.705933
GCACGCA850.093.840882
GAGCATC3200.092.896034
CAGGTAT259.379113E-487.01611
AGCATCT4000.087.0050665
GTAACAA11600.082.504794
AAACACG2700.080.5602345
CGCACTT1000.079.754655
GAAACAC2750.079.0955054
ACGTGCA300.001934170672.513412
GCGGAGC608.019197E-872.513411
ACGCACT1100.072.504214
TAGTAAC13650.071.716562
TAACAAG13500.070.8930055
TCTAGGG754.8512447E-967.67065
Produced by FastQC (version 0.11.2)