FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_445_rr.351000000a99a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_445_rr.351000000a99a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141911
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA95500.44586353027740183No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC53640.2504305734458621No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT38630.18035296517922547No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC32670.152527345907463No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT30860.14407694810848817No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC30090.14048202749787458No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG30080.14043534021721724No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT28110.1312379459277253No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA27350.127689712597769No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA26150.12208723891889066No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT25310.11816550734367581No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTTATTGAATTAATGGAT24680.11522420866226467No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT23870.1114425389290218No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT22970.10724068366986304No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG22830.10658706174066057No Hit
GTATTACCCAAGGGTTGTTACTAATACATTCTTCTATTCCAGAATACATT21820.10187164639427128No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT21510.10042434069389439No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC100.0070916084144.99873145
AGCACGC500.0130.50191
AGGATTG11850.0129.703525
CGGTGCA6850.0119.59752
GAGCAGA2700.0115.4646451
CTAACTT203.8668007E-4108.749054145
GTAACAA25750.0100.7952354
GAGCATC10250.098.316224
GAAACAC11750.098.105524
GCACGCA700.093.213472
CGGAGCA2600.092.0184252
AGCATCT12400.090.0395365
GGATTGA17000.089.131586
TTCGGAA259.388547E-486.99924145
CGGAGCC502.2700988E-886.999242
GAGTTGT1100.085.6830751
GCGGAGC3400.085.2953641
CACGCAC801.8189894E-1281.561793
TAGAAAC16150.079.457512
AACACGT1750.078.71366
Produced by FastQC (version 0.11.2)