FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_443_rr.351000000a9994.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_443_rr.351000000a9994.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1687072
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT37250.22079674133646934No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG30540.18102369074941674No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA29670.17586682726048444No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT29010.17195472392405303No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA27610.16365632290738036No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC23280.1379905540486713No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA23200.13751635970486142No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG20790.1232312550975892No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC19900.11795584302270444No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA19740.11700745433508468No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC19130.11339172246353445No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG19020.11273970524079589No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG18360.1088276019043645No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT17880.10598243584150528No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA17520.10384856129436089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT3800.0110.660695
ACGCACT3950.0108.293894
AGCACGC3750.0108.272621
GCACGCA4100.099.026892
CACGCAC4250.098.943693
GTAACAA27250.097.9107364
CGGTGCA4050.096.669112
CGTGCAC1300.094.81013
TAGTAAC31450.085.526812
AAACACG11300.084.0508045
TAACAAG32650.081.495185
ACGTGCA3400.078.8990552
GGTGCAT4600.077.230223
ACGAGCG950.076.315469
ACAAGAG37150.071.62157
GAAACAC13450.070.615174
AGTAACA38850.069.982473
AACACGA13050.064.446086
CGGAGCA1250.063.8016132
TAGAAAC18200.061.7460672
Produced by FastQC (version 0.11.2)