FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_436_rr.351000000a9987.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_436_rr.351000000a9987.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1772696
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT39630.2235577899425508No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA35400.1996958305315745No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG32900.1855930176409266No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT31060.17521334735340974No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA26160.1475718340877398No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC26120.14734618908148944No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG25590.14435639274867207No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC22480.12681249351270607No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT21880.12342781841895058No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG21620.12196112587832318No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT20990.11840721702987991No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG20600.11620717821893883No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG19950.11254044686737037No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC19830.11186351184861928No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT19010.10723778922048677No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC18820.10616597544079753No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT18620.10503775040954569No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA18420.10390952537829386No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA17890.1009197290454765No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG100.00709109145.001573
CGCACTT4350.0116.667945
CGGAGCG203.8659538E-4108.754252
GTAACAA29300.093.038554
AGCACGC5550.091.4579241
TAGTAAC30750.089.5972
CGGTGCA5800.081.2531742
TAACAAG35100.078.0777745
GCACGCA6850.075.1489262
ACGCACT7300.071.507624
GCGCTAA451.19404765E-464.4451456
AGTAACA43000.064.2390753
ACAAGAG45450.059.6579327
ACAGCGC502.010562E-458.0006263
AGCGCTA553.2195094E-452.7278445
AAACACG14250.051.89535
AACACGA13000.051.308256
GAAACAC15450.048.803124
ACGAGCG604.945856E-448.333864
GTAGTAA60700.048.0234831
Produced by FastQC (version 0.11.2)