FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_434_rr.351000000a997a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_434_rr.351000000a997a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1261120
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC43280.3431870083735093No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA27650.21924955595026643No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC25530.20243910175082466No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT25450.20180474498858159No Hit
GGATACAACTTAAAACGAATGCTGAAGACATAGGAACCAAAGGCCAAATG19610.15549670134483634No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT18650.1478844201979193No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT17310.1372589444303476No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC16980.1346422227860949No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT16230.12869512814006598No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC15070.11949695508754123No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT14520.11513575234712002No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG14420.11434280639431617No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA13820.10958513067749302No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT13580.10768206039076376No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGCA100.0070894966145.010833
TCGGAAT100.0070911776144.99933145
ATGCCGC100.0070911776144.99933145
GTAACAA14550.0117.100334
CGGTGCA5150.0115.445512
GAAACAC5900.0104.452814
CGCACTT1150.0100.873095
AAACACG6450.095.5459755
GCGGAGC1850.094.0648041
CACGCAC1250.092.806933
AGCACGC1300.089.240971
TAGTAAC19400.088.949942
CGGAGCA1650.087.8853452
TAGAAAC7350.087.795672
ACGCACT1350.085.928934
GCACGCA1400.082.863332
AACACGA7300.080.448026
GAGCAGA2250.077.342181
GAGCATC7700.077.2104954
TAACAAG22250.076.9015665
Produced by FastQC (version 0.11.2)