FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_428_rr.351000000a996d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_428_rr.351000000a996d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781333
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT10250.1311860627926889No Hit
ACAACAGCTTGTGCAAAGGCCCTGAGGCATGAATGATTAAAAGATATAAT9980.1277304299191254No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC7980.10213314937421047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC15800.0107.845462
GTAGTAA17150.0100.61811
GTAACAA18150.095.080494
CGCACTT5350.093.5161065
GCACGCA6500.079.201992
TAACAAG22550.076.206665
AGTAACA25400.068.2268453
ACGAGCG554.060912E-665.9087149
TCTCGCT4000.061.6325382
AAGAGCG3900.061.3458029
CTCGCTC4150.059.404853
TCGCTCT4550.057.369654
ACAAGAG30550.056.247147
AACAAGA33750.051.3470156
ACGCACT10300.049.9818424
CGCTCTG5100.049.7609865
GTCTCGC5200.048.800921
CGAGCCG604.941433E-448.3392455
AGCACGC10750.047.2120551
CAAGAGC33000.045.9193428
Produced by FastQC (version 0.11.2)