FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_425_rr.351000000a9951.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_425_rr.351000000a9951.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1245541
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT33670.27032430084597775No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT28320.2273710781098334No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG25500.20473031397601527No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24580.19734396539335114No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA23780.19092105358233893No Hit
ATATTGTAGTTGGGTCTCTACAGTGAGGCAGGATCTGCCCAGGCAGAGGG19790.15888678092491537No Hit
CCTAAGGGGTAGCCACTACTCAGCACCCACTTTTTGTTGCCAGACCTTCC18050.14491694773596372No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA17860.14339150618084834No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC17800.14290978779502242No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG17270.1386546087202268No Hit
CCCCTTAGGTGGACCCTAAACTCTTCACTATAACTTCCCTGCCCAGCACC17130.13753059915329965No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG16980.13632630318873487No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT16720.13423885685015588No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG16410.13174997852338863No Hit
CAGTAAAGTCTGAGCTAAAATCGGAGGCTCTCGAGCCACATCCACCTCGC15880.12749479944859302No Hit
CATTTAGGTTTCTGGTCTCTGATAATTTGGAAGGTCTGGCAACAAAAAGT15860.1273342266533177No Hit
CCCATATTCCAGGCCCTCCCACAGCCCTCACCCGGTCAGTCACCCCATCT15800.1268525082674918No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGAAATAGTATGTTATCATC15470.12420305714544926No Hit
GTAGTAACAAGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC14860.11930558688955241No Hit
TCACAGAGCACCCCCTAGTCTTGGAAACCCTTCCTCAAAGAGTCACCTGG14800.11882386850372649No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG14740.11834215011790057No Hit
CTACAGTGAGGCAGGATCTGCCCAGGCAGAGGGAAGAGCAGGCAGAGCCG14050.11280238868090252No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT13830.11103608793287414No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT13530.10862749600374455No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA13520.1085472096061069No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC13190.10589775848406435No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG13160.1056568992911514No Hit
GTAGAAACACGAGCGTACATCAGAATCCCAGGAGGGATGGCGTGCTGGAC13160.1056568992911514No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA13150.10557661289351374No Hit
AATTAAAACAAGGGGTGATGGGAAGAATCACAGGTTGTGGCCCCGGGGTT12990.10429203053131131No Hit
GTCCAGAAGAGGGTGAAAGGAACCTCCACTTCGGCCATTGTGGAGGCTTA12970.10413145773603598No Hit
GGCTTAGATAAGTAGTTCCAATACCAGGTGCAATAGGGGAAGGAAAAAGT12750.10236515698800763No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC12660.10164257940926875No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG12540.10067914263761692No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT12530.10059885623997925No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT3050.092.711855
GCACGCA3050.090.3346252
GTAACAA15500.080.92544
CCGTTCA502.3066495E-672.49969
GAGCATC3900.065.068974
TAACAAG19500.064.3253255
TAGTAAC20450.064.173512
AGCACGC4300.064.071981
AAACACG7500.062.8380285
ACACGAG7000.059.0377587
AGTAACA23150.055.7493063
GAAACAC8550.055.121084
AGCATCT5200.054.3790665
ACGAGCG3900.053.9099589
CACGAGC7700.053.670698
AACACGA7750.053.3265656
ACAAGAG24000.052.8664177
ACGAGCC1258.913048E-1152.199719
CGGTGCA2200.049.4355162
ATTAGTA7000.048.6802561
Produced by FastQC (version 0.11.2)