FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_421_rr.351000000a9944.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_421_rr.351000000a9944.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences940257
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACAGCTTGTGCAAAGGCCCTGAGGCATGAATGATTAAAAGATATAAT22860.24312501794722083No Hit
GTTATAACCTGTGTTTGAGAAAGGCTAGCTCTGTTTCTATTGCCATAATT15210.16176428359480438No Hit
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT14260.15166066298894876No Hit
ACAACAGCCAACATAGATACCACAGGGCACAAAAGGCCAGGAATACAAAA13190.1402807955697219No Hit
GCGGTGCAGACCACAAACAGAATAAACAAAAGGTAAATTGTTGGACTTAA11780.12528489551260985No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG11060.11762741463238244No Hit
GTCTCGCTCTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCAC10750.11433044369784007No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA10440.1110334727632977No Hit
TCTGTATACCCTCTAATGAAAATTTTTTAAAGTACCAAGAAATTAAAAAG9550.10156797556412768No Hit
GTGCAGAAGCTTTGAGCTTAGTGGATGCAGAGGGCGTTGTGAATCAGAGC9430.10029172875075645No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT5550.0121.485555
TAGTAAC15200.0110.657042
GTAGTAA16050.0105.2596051
GTAACAA16800.099.686744
TAACAAG18950.088.759225
GCACGCA8650.078.785522
AGCACGC9350.072.89491
AAGAGCG4450.068.4264459
AGTAACA25500.065.960273
CGGTGCA5700.059.7802432
ACGCACT11600.059.3745424
TCTCGCT4900.059.1832122
AACACGA10100.056.713521
ACGAGCA6200.056.12862
CTCGCTC4950.055.6561363
TCGCTCT5250.053.8567284
ACAAGAG31500.053.1662567
CGCTCTG5350.051.494935
CGAGCAT3950.051.3920063
AACAAGA32900.050.9038666