FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_420_rr.351000000a9937.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_420_rr.351000000a9937.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1459326
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGTGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT30500.20900059342463576No Hit
GCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACAAT26470.18138510517869208No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT23270.15945717406528767No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT22860.15664765789138274No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC21520.14746533673764464No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG20880.14307975051496374No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA20290.13903678821592982No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT18750.12848397136760395No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT15660.10730981288622282No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC15570.10669308982365833No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC15220.10429472235812971No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT14660.10045733441328393No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGA100.007090753145.003022
CGCACTT1700.0115.145515
CGGTGCA7050.095.640292
AGCACGC2200.088.988271
GAAACAC8750.086.9988254
AAACACG8850.086.015795
GGTGCAT7750.084.1953053
GCACGCA2400.081.564192
GCGGTGC11000.073.827311
AACACGA10950.066.209156
ACGCACT3100.063.1443144
CTCGCTC954.5656634E-1061.0539053
AGCGTTT502.0106854E-457.9992185
ACACGAG12750.056.861987
GTAACAA27550.054.999264
TAGTAAC28600.053.4888762
CACGCAC3750.052.2010843
GTAGTAA30050.051.878291
TAACAAG29500.051.3637165
CACGAGC14700.049.319068
Produced by FastQC (version 0.11.2)