FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_418_rr.351000000a992a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_418_rr.351000000a992a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4374610
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA111350.2545369758675631No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT110110.25170243747442633No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG93070.21275039374938567No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC73280.16751207536214657No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG71650.16378602892600713No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC67980.1553967096495459No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA67500.1542994689812349No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT65110.1488361248202697No Hit
TGATACAACCATATCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT60460.13820660584600686No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG59740.13656074484354033No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG59710.1364921673017709No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC58320.13331474119978695No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT56130.128308580650618No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG55760.12746279096879493No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA53880.12316526501791017No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA51190.1170161454392506No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG51190.1170161454392506No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC50780.11607891903506827No Hit
GTTTCTAATAATGATAATGATACAACCATATCAGCTATTATGGAGCTGTT48890.11175853390359368No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC47900.10949547502520224No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG47630.10887827714927731No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT47450.10846681189866068No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA47420.10839823435689125No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG46880.10716383860504136No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA45660.10437501857308423No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA44880.10259200248707884No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC44410.10151762099935766No Hit
ACACCAGAGAGGTGCCTTGATGACATAGAGGAAGAACCAGAGGATGTTGA43780.10007749262219946No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC9100.0108.3626861
CGCACTT9650.0103.684585
CGGTGCA8100.098.463442
GTAACAA64500.093.749454
TAGTAAC69500.087.11022
ACGCACT11700.086.1373144
CGGAGCG300.00193566272.50492
GCACGCA14400.068.9803542
GAAACAC25050.068.5966644
TAACAAG89200.067.789685
AGTAACA92600.066.084373
TATCGGG350.00356748462.142086145
GAGCATC19400.060.5446364
AACACGT4350.060.0033726
ACAAGAG103850.058.0145657
AGCATCT22050.057.8717275
AAACACG29450.057.6093445
AACACGA25250.057.141826
ACACGTG4450.057.0230677
CGGAAAA7250.057.0058021
Produced by FastQC (version 0.11.2)