FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_412_rr.351000000a991d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_412_rr.351000000a991d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172096
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG83170.3829020448451634No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG39170.18033272930846517No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT37320.17181561035976312No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT35990.16569249241285838No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA35560.16371283773829517No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG32740.15072998615162497No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG29640.13645805710244852No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA28820.13268290167653732No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG28030.1290458616930375No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT27020.12439597513185421No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG24580.11316258581572823No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGT24510.11284031645010166No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA24480.11270220100769027No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT24300.11187350835322196No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC24230.11155123898759539No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG24090.11090670025634226No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT23910.11007800760187395No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC23720.10920327646660184No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC23540.10837458381213352No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG22500.10358658180853884No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC22290.10261977371165915No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC22280.10257373523085535No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA7150.0117.6245962
GGTGCAT9000.093.4462053
AGCACGC3650.093.364381
CGCACTT3950.088.1029365
ACGCACT4050.085.927554
GCACGCA4400.079.092412
GCGGTGC13150.074.43621
GCGGAGC3550.073.5275961
GAAACAC11550.073.442954
ACAACGC502.3072098E-672.4996958
GTAACAA36750.071.810884
AAACACG13200.065.910345
ACGTGCA1600.063.4386982
TAACAAG42700.062.3138245
GACCGAC350.003566799262.144032
CCGACCT350.003566799262.144034
ATTCGGT350.003567124762.142597145
GCGCTGT350.003567124762.142597145
AACACGA12800.061.173036
GCAGAGC24800.060.81181
Produced by FastQC (version 0.11.2)