FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_403_rr.351000000a98e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_403_rr.351000000a98e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346205
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT29210.21698032617617674No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA22930.1703306702916718No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT20460.1519827960823203No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA18760.1393547045212282No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18240.13549199416136473No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT17530.13021790886232038No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG17140.12732087609242276No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC16590.12323531705795181No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC14650.10882443609999963No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT14560.1081558900761771No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC14290.10615025200470954No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC14170.1052588573062795No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA14010.10407033104170613No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA13520.10043046935645017No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT13500.10028190357337849No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT2400.0105.728415
ACGCACT2500.0104.399254
AGCACGC2450.0103.574531
GTAACAA16150.096.5163354
CGGTGCA2600.094.807022
GAAACAC6700.084.402384
GCACGCA3150.080.554982
AAACACG7900.071.5817645
CGGAGCA2450.071.01992
GAGCAGA4700.070.959571
TAACAAG22550.069.445185
TAGTAAC23750.066.241632
AACACGA8300.065.511586
GGTGCAT3800.062.9600753
GCGGAGC4050.060.8660241
TAGAAAC11200.057.6111952
ACACGAG9600.056.6402177
ACAAGAG28400.054.6298877
AGAAGCG2850.053.4206665
CACGCAC4850.052.3192143
Produced by FastQC (version 0.11.2)