FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_402_rr.351000000a98d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_402_rr.351000000a98d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1843962
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT38480.2086810899573852No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT30630.1661097137576588No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA29110.15786659378013213No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG27850.1510334811671824No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC24600.13340838910997083No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA23530.12760566649421193No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT22250.12066409177629474No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG21660.11746445967975479No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC21580.11703061125988495No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC20640.11193289232641454No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC19670.10667248023549293No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG19620.10640132497307428No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT19090.1035270791914367No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA18540.10054437130483165No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCA100.0070908912145.00314
GCACGCA553.6379788E-12105.456792
GAGCAGA3600.0100.6993261
CACAGGA3500.099.4306953
AGCACGC607.2759576E-1296.671351
CAGGATT3600.096.6687245
ACACGTG451.0923031E-896.666117
GTAACAA16100.090.064034
CGCACTT651.6370905E-1189.232675
AAACACG6950.083.455025
CGGTGCA2900.080.001712
GGTGCAT2900.077.5016563
CACGCAC755.638867E-1177.334983
GAAACAC7500.077.334984
AACACGA7350.072.994756
ACGTGCA809.822543E-1172.501552
CGGAGCA2400.072.501552
ACGCACT851.6916601E-1068.236754
CAGCATC851.6916601E-1068.236755
AACACGT651.3985118E-766.924516
Produced by FastQC (version 0.11.2)