FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_398_rr.351000000a98cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_398_rr.351000000a98cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66871
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTTGATGA2070.3095512254938613No Hit
GAATAAGCAGGAGTATTGCTGTGGTGGAAAAGGACCCTCTGGTGAAGCTT1810.2706703952385937No Hit
AGCTTATACAATTCTGACTGAAAAATTAAAGTTGTGCAAACTTTCCACTT1800.2691749786903142No Hit
TTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTTAAGATTAGGA1490.22281706569364898No Hit
GAGTAACACCTGGGAAAGCTTCACCAGAGGGTCCTTTTCCACCACAGCAA1420.2123491498556923No Hit
GGCTAACCTTCTCAAAACGCACTCATAATGAGCAGATGTTATCATTCTTT1370.20487206711429468No Hit
CATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTAACA1370.20487206711429468No Hit
GTTTACAGCAGTGAGGAGAGAGGGAAAACTTGTGGAAAAACTGAATTACA1310.19589956782461757No Hit
TGTCAATGCAATCTTTTTTACATTATTAACTGAAGAAAAATGGGGCCCTT1300.19440415127633803No Hit
GTCAAGAGCAAAGATCATGGCAACATTTTGGGATGCTCAAGGCATTTTGC1280.191413318179779No Hit
GTGTTACTCTGCTCATGCTCTGGCTAACCTTCTCAAAACGCACTCATAAT1280.191413318179779No Hit
CTTTTATAAGTGGTACTACCAGCCACCTAATTCATCCCTAAAAAACTAAG1260.18842248508321993No Hit
TTCTTTCACCCTCCAGAAAGTCAAAAAGCAAAATGCCTTGAGCATCCCAA1230.18393623543838136No Hit
GCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAAA1210.18094540234182233No Hit
GCTTATTCTCATCAAATGTAGGCAACTTTTCAAGAGTTTTGATGGGAAAT1200.1794499857935428No Hit
AATCAGGACTGAAAGGTGGATGCCCAATGATTTCCCATCAAAACTCTTGA1200.1794499857935428No Hit
ACATAATGAACATAAAAAATGTGAACTTTATTTTTTTAACTTAAGTTCCA1090.16300040376246802No Hit
GGCTTTACCAGTATGATTCTGAAGACAAAGCACAATCAAAGCAATGGCTA1080.16150498721418852No Hit
GCTTTCAATGGAAATTTTAAACAAGGGGGATTAAGATCCTGAAGCACTTC1040.15552332102107042No Hit
CTTCTCAACTGGTAGCTTATACAATTCTGACTGAAAAATTAAAGTTGTGC1030.15402790447279088No Hit
GCTCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAAGAATGATAAC1020.15253248792451138No Hit
CTCATAATGAGCAGATGTTATCATTCTTTCACCCTCCAGAAAGTCAAAAA1010.15103707137623185No Hit
GCCTTGAGCATCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCTG1000.14954165482795231No Hit
GGTTAGCCAGAGCATGAGCAGAGTAACACCTGGGAAAGCTTCACCAGAGG1000.14954165482795231No Hit
TGCTTATTCTCATCAAATGTAGGCAACTTTTCAAGAGTTTTGATGGGAAA1000.14954165482795231No Hit
GCTCATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTA990.1480462382796728No Hit
GTCAAAGCAAATGCAGACCAGTCAAGAGCAAAGATCATGGCAACATTTTG970.14505540518311377No Hit
GTTCTGAAATAACAAAATTTTATTTAAAAATGGGAAAGATTAGTTTACAG970.14505540518311377No Hit
TCCCAAAATGTTGCCATGATCTTTGCTCTTGACTGGTCTGCATTTGCTTT920.13757832244171614No Hit
ACCTAATTCATCCCTAAAAAACTAAGGGTCCTGGGAATTTAACCATGTCA910.1360829058934366No Hit
TTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCATGAGCAGAGTAACACC900.1345874893451571No Hit
AGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAA900.1345874893451571No Hit
GTATGATTCTGAAGACAAAGCACAATCAAAGCAATGGCTACCAAGAGGTG890.13309207279687757No Hit
GTCAGAAGGAGACAAATCAGGACTGAAAGGTGGATGCCCAATGATTTCCC880.13159665624859807No Hit
AATTAAAGTTGTGCAAACTTTCCACTTGATGAGTGCCGAAACCATTGTGT880.13159665624859807No Hit
CTTTTTTACATTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTT880.13159665624859807No Hit
TCTGAAGACAAAGCACAATCAAAGCAATGGCTACCAAGAGGTGGAAATAG860.128605823152039No Hit
GTGCAAACTTTCCACTTGATGAGTGCCGAAACCATTGTGTCCAGCTCAGC860.128605823152039No Hit
GGTAAAGCCATGTCCTATTGCAGTTCTTCCAAGAAGTGCTTCAGGATCTT840.12561499005547996No Hit
ATTTTGGGATGCTCAAGGCATTTTGCTTTTTGACTTTCTGGAGGGTGAAA840.12561499005547996No Hit
GCTCATGCTCTGGCTAACCTTCTCAAAACGCACTCATAATGAGCAGATGT830.12411957350720045No Hit
GGTGGAAAAGGACCCTCTGGTGAAGCTTTCCCAGGTGTTACTCTGCTCAT770.1151470742175233No Hit
TTTTTACATTATTAACTGAAGAAAAATGGGGCCCTTTACAGATTTTTTAA770.1151470742175233No Hit
GTACTACCAGCCACCTAATTCATCCCTAAAAAACTAAGGGTCCTGGGAAT770.1151470742175233No Hit
GTCTTGGCTAAGGGAAGCCCAGGGAAAGGTTTTGATCTTTACTTCAGCCA770.1151470742175233No Hit
GCATTGACATGGTTAAATTCCCAGGACCCTTAGTTTTTTAGGGATGAATT760.11365165766924377No Hit
TCTGGAGGGTGAAAGAATGATAACATCTGCTCATTATGAGTGCGTTTTGA760.11365165766924377No Hit
CTGGTGAAGCTTTCCCAGGTGTTACTCTGCTCATGCTCTGGCTAACCTTC750.11215624112096426No Hit
GTAGAAACAACAGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAA750.11215624112096426No Hit
TCACCAGAGGGTCCTTTTCCACCACAGCAATACTCCTGCTTATTCTCATC730.10916540802440519No Hit
GATAACATCTGCTCATTATGAGTGCGTTTTGAGAAGGTTAGCCAGAGCAT730.10916540802440519No Hit
ATCAAAGCAATGGCTACCAAGAGGTGGAAATAGCCCAGTCAAAGCAAATG720.10766999147612567No Hit
AAACAACAGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCT720.10766999147612567No Hit
CATAATGAGCAGATGTTATCATTCTTTCACCCTCCAGAAAGTCAAAAAGC720.10766999147612567No Hit
GAAAGGTGGATGCCCAATGATTTCCCATCAAAACTCTTGAAAAGTTGCCT710.10617457492784615No Hit
GAATTAGGTGGCTGGTAGTACCACTTATAAAAGTGTCTTGAACTTGATGG700.10467915837956664No Hit
CTTAAGTTCCATCAAGTTCAAGACACTTTTATAAGTGGTACTACCAGCCA690.10318374183128709No Hit
GATGTTATCATTCTTTCACCCTCCAGAAAGTCAAAAAGCAAAATGCCTTG680.10168832528300757No Hit
CCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAAAAATTAAA680.10168832528300757No Hit
CACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGACTGAAAAATTA680.10168832528300757No Hit
CTTCTTGGAAGAACTGCAATAGGACATGGCTTTACCAGTATGATTCTGAA680.10168832528300757No Hit
AAGCAGAGCAGACCACAAACAGAATAAACAAAAGGTAAATTGTTGGACTT680.10168832528300757No Hit
CCTTTACAGATTTTTTAAGATTAGGAAACAAGAAGTCAGAAGGAGACAAA670.10019290873472805No Hit
GAAGAAAAATGGGGCCCTTTACAGATTTTTTAAGATTAGGAAACAAGAAG670.10019290873472805No Hit
TTCGTATTCCATGTGAGAACAGAGAGTTATATTAGTGAGAGACAGAAAGA670.10019290873472805No Hit
TCCAAGAAGTGCTTCAGGATCTTAATCCCCCTTGTTTAAAATTTCCATTG670.10019290873472805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCC100.007076174145.04
TTTACGG100.007076174145.02
ATAATAG100.007076174145.04
AGTTATC100.007076174145.01
TTACGGG100.007076174145.03
TTTTACG100.007076174145.01
TACGGGT151.2249172E-4144.999984
ACGGGTG151.2249172E-4144.999985
AAGAGCG406.054852E-790.6259
CAGAAAT259.3482016E-487.02
ACGAGCT259.3482016E-487.09
AATCTCG300.001927823172.499996
TAACAAG902.746674E-1064.444445
TAGTAAC902.746674E-1064.444442
ACTTGAC350.003551966562.1428578
GCACGCA350.003551966562.1428572
CGCACTT350.003551966562.1428575
GCAGAAG702.3014582E-762.1428571
GTAGTAA1153.8198777E-1156.739131
GGAGTTG400.006026313654.3756
Produced by FastQC (version 0.11.2)