FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_395_rr.351000000a98be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_395_rr.351000000a98be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131757
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT43300.20311883577724854No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT38370.17999237248898445No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC35650.1672329444678732No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT29260.13725767055063032No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA29060.1363194773137839No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT28180.13219142707165968No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC27060.12693754494531975No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC26280.12327859132161875No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC25420.11924436040317916No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC25060.11755561257685561No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT24780.11624214204527064No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA24380.1143657555715778No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT23940.1123017304505157No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT23090.10831440919391844No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT22310.10465545557021742No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA22200.10413944928995192No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG21910.1027790690965246No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT21490.10080886329914714No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT21420.10048049566625089No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGC100.0070910538145.002528
TACACGG100.007091551144.99911145
GTAACAA31200.0118.746824
CGGTGCA5150.0114.0364462
CGCACTT2200.0108.754445
AGCACGC2150.0107.91391
GAAACAC11200.097.749534
AAACACG11600.094.3788455
ACGCACT2600.092.022984
CGGAGCA5900.090.938042
TAGAAAC13700.090.498512
GCACGCA2600.089.2364962
GGTGCAT6600.088.982993
GCGGAGC7800.087.37741
TAGTAAC43600.087.1386262
TAACAAG43900.084.228955
CGGAGCG755.638867E-1177.33832
ACAAGAG48950.075.537577
GAGCAGA4550.073.3014141
CAGCGTT300.001935730772.502965
Produced by FastQC (version 0.11.2)