FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_394_rr.351000000a98a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_394_rr.351000000a98a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800796
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT75540.2697090398586688No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT72990.2606044852963229No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA67950.2426096009848629No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG66320.23678982689206923No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC55830.19933618871206615No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC55290.19740816539298112No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT53310.19033874655633612No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG51680.1845189724635425No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA48640.1736649152598047No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT45840.16366775730899358No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC42710.1524923628854083No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG42600.15209961739448358No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG41990.1499216651266283No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG41180.1470296301480008No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA39560.14124556019074577No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA38800.13853204588981133No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT36760.13124840223993464No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC35600.12710672251745575No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT34940.12475024957190742No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC34880.12453602475867576No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG34220.12217955181312741No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC33850.12085849879819881No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA33290.11885906720803657No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA33280.11882336307249795No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG32510.11607414463602489No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT32030.11436034613017156No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAA31640.11296788484416573No Hit
GTTTCTAATAATGATAATGATACAACCATGTCAGCTATTATGGAGCTGTT31570.11271795589539546No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT31560.11268225175985684No Hit
GCTGTACACTTCAACCACATCCTTCATTTCATACAATGTTTCTAATAATG31500.11246802694662517No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT31210.11143260701600545No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT29560.1055414246521346No Hit
AATAGCAACTGTACGAAATACAATTGGACCGATTACCCTTCAACACCAGA29220.10432748404382182No Hit
AAATACAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGA29100.10389903441735848No Hit
GATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGTG28400.10139974492965571No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA21900.069.522684
CGTCAGA350.003566740862.144765
TAACAAG25800.059.0134355
GAAACAC9250.058.7855834
AAACACG9250.058.7855835
CACGAGC8850.057.3453678
TAGTAAC29950.052.04762
GAGCAGA11000.046.8002321
AAGCGGA2050.045.9778442
ACGAGCG803.7365004E-545.3122679
GAGCGTT657.3383923E-444.6159557
AACACGA11950.043.683356
ACGAGCC3400.042.646849
AGAAGCG5700.041.9749685
ACGAGCA7150.041.5732159
GCACTTA1401.41571945E-841.429846
GTGCCCG11050.041.334639
AGCGGAG2300.040.9802473
ACACGAG12950.040.3093957
ACGAGCT1105.267886E-639.5452549
Produced by FastQC (version 0.11.2)