FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_390_rr.351000000a9895.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_390_rr.351000000a9895.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797111
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT34160.19008286076931252No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24860.1383331357940606No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT23210.1291517329758707No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC20920.11640905876153448No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG20920.11640905876153448No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC20830.11590825497145141No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT20680.1150735819879796No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA20550.11435019873563737No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG20280.11284778736538811No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGCTCTTCCGTGACC19250.10711636621221504No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC18870.10500186132075315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA3750.094.740361
GTAACAA18850.092.699174
CGGTGCA3250.089.239862
CAGGATT2750.084.369895
AAACACG7200.083.582595
GAAACAC8450.072.076344
CACAGGA3400.070.3728563
GCACGCA851.6916601E-1068.242252
AGCACGC851.6916601E-1068.240351
TAACAAG26450.066.063495
AGAAGCG4250.063.122325
CGCACTT954.5656634E-1061.057155
AACACGA9200.059.895746
CACGCAC1007.203198E-1058.004293
TAGTAAC31200.057.8664782
TAGAAAC11400.056.6066442
ACGCACT1051.1095835E-955.2421884
TAGTAGA3200.054.379033
ACACGAG10200.054.0221067
GAGCATC6350.053.665394
Produced by FastQC (version 0.11.2)