FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_385_rr.351000000a9888.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_385_rr.351000000a9888.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293503
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA40290.3114797569081788No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG38640.29872369836018936No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG38560.29810522279422624No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT34320.2653260177981806No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC33430.25844547712684085No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT32380.2503279853235748No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT31880.24646251303630531No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC30200.23347452615107966No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG29110.22504779656483206No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAA27300.2110547868849164No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT26910.20803971850084615No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC26320.2034784612018681No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA25790.19938106057736238No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT25690.1986079661199085No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA25450.19675253942201912No Hit
CTTCAAGGGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT23420.18105872193570482No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG23300.18013100858676015No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG23140.1788940574548339No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA22430.17340508680691116No Hit
TCCGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT21940.169616923965387No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT21660.16745225948451609No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG21010.16242714551106568No Hit
ATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCATC20890.16149943216212098No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT20850.16119019437913945No Hit
GTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATGACCACA18490.14294516518322725No Hit
AAATACAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGA18460.14271323684599108No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGTTTGACA17890.1383065984385038No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG17710.13691502841508676No Hit
AATAGCAACTGTACGAAATACAATTGGACCGATTACCCTTCAACACCAGA17660.13652848118635982No Hit
TTCCTCAAACACCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATT17620.13621924340337827No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG17130.13243108056185413No Hit
GGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCAGAG16910.13073027275545554No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG16830.1301117971894924No Hit
GTTTAGGCGGTCTTGACCGGGGTAGTCAAGGGCTCTTTGCCATGAAAGCC16720.12926139328629313No Hit
GAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGATCTTACAGTGG16680.12895215550331154No Hit
ATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTC16470.12732865714265837No Hit
GAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACA16260.12570515878200514No Hit
CAATTGGACCGATTACCCTTCAACACCAGAGAGGTGCCTTGATGACATAG16160.12493206432455123No Hit
TCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTTATTCTTGACTCT15860.12261278095218953No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC15700.12137582982026326No Hit
ATACTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGA15690.12129852037451787No Hit
CATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTCA15640.12091197314579093No Hit
ACTCTGCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAA15550.12021618813408241No Hit
GCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCCCGGTCAAGAC15360.11874730866491999No Hit
CCTATTTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTA15310.11836076143619303No Hit
TTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGAAC15300.11828345199044765No Hit
CCTTTATCTTTTGCCTTGCATCTTTGTTGTTCATGTCCCTTAATACTATT15180.11735573864150295No Hit
CTATTATGGAGCTGTTAGCTATCACTGTTTTGTGGAATAGTATGTTATCA15170.11727842919575755No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCCTCTGGTTCTTCCTCTA15130.11696919141277601No Hit
GATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCC14990.11588685917234053No Hit
GGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCGAACAACAACATG14930.11542300249786819No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA14890.11511376471488664No Hit
AGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGAGAAAAATGGCG14750.11403143247445116No Hit
CCCCAATGGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATG14230.11001134129569085No Hit
ATTCTGGAGTGCTATGAAAGGCTTTCATGGCAAAGAGCCCTTGACTACCC14180.1096247940669639No Hit
GTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACCAGTC14150.10939286572972773No Hit
GAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCG14030.10846515238078303No Hit
GGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACCGCAGT13940.10776936736907451No Hit
CTTCTGGTGATAATCGGTGCTCTTGACCAAATTGGGATAAGACTCCCACC13830.10691896346587523No Hit
GAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGAAGTAAACACTCAGA13750.10630048789991212No Hit
GCGTAATGTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAAC13540.1046769895392589No Hit
ATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTG13370.10336272896158726No Hit
GGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTT13350.10320811007009648No Hit
GTATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAA13320.1029761817328603No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAG13280.10266694394987874No Hit
GCATAGATTGAATGCATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTG13210.10212577782966101No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG100.0070895916145.010331
GGAGCGT100.0070912302144.999118
TCGGTGG100.0070912302144.999114
AGCCGTT353.437764E-582.856637
GCCGTTC353.437764E-582.856638
CCGTTCA353.437764E-582.856639
CGCTGTG300.00193591872.499569
GAGCCGT406.6641274E-572.499566
TAGTAAC13350.062.4528052
GTAACAA13750.059.5814554
GCGTAGA902.0503649E-856.3885422
AACACGA6350.055.9445346
ACGAGCG400.00605143254.374679
ACACGAG7300.048.6640857
CGATGCA450.00964000448.333049
GAAACAC8200.047.743614
TAACAAG18700.044.197595
ACAAGAG18800.043.5768627
GTAGAAA12700.042.8180051
AAACACG9500.041.9734275
Produced by FastQC (version 0.11.2)