FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_383_rr.351000000a987b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_383_rr.351000000a987b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1822758
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCATTATGACTTCC45370.24890852214062426No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT27770.15235154639288373No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT24960.13693534742406838No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG21190.11625240432355803No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA20630.11318013691340265No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC19400.10643212099466853No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA18620.10215289138766638No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG8500.084.44025
AACACGA8500.079.322626
ACACGAG8550.078.8587347
GTAACAA15200.077.2688454
GAAACAC10700.068.433794
CACGAGC10050.067.0887768
CGGGTAC350.003566749962.143845145
ACGAGCC3950.060.5689249
CGGTGCA1800.060.415972
GCACGCA2100.058.68982
GAGCAGA9100.057.363551
CGCACTT2150.057.3249175
AGAAGCG5000.055.099365
TAGAAAC14850.054.67952
TAACAAG22250.052.7859085
AGCACGC2350.052.4476431
TAGTAAC23350.051.851652
ACGCACT2450.050.3055424
TACAGCG752.5515908E-548.3327755
CGGAGCA2850.045.7889442
Produced by FastQC (version 0.11.2)