FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_380_rr.351000000a986e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_380_rr.351000000a986e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005663
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA17030.16934102179358293No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT15880.15790577957029345No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG14100.14020601334641922No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT13760.13682515912388146No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC13350.1327482466790565No Hit
CTATAGAACATGCTGGTGAATTCAAACATTCCAGTTTCGTTGCAGTAACT13230.13155500401227846No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG12840.12767696534524986No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT12390.12320230534483222No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG11690.11624172312196034No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT11170.11107100489925552No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC10460.10401098578748547No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG10060.10003351023155868No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCCG100.007090948144.99945145
CGCACTT1700.098.092745
CGGTGCA3050.097.463492
GCACGCA1650.096.671112
AAACACG7550.096.030915
GAAACAC7800.093.8825154
ACGCACT1950.089.234864
AGCACGC1800.088.62841
ACGAGCG259.3869044E-486.999679
GTAACAA21350.083.540144
CACGCAC2000.079.753663
TAACAAG24350.072.652215
GCATTCG300.001935774672.4997259
GCGGTGC6100.071.325391
AACACGA9400.070.18946
TAGTAAC25500.069.375742
GTAGTAA28150.063.3693771
ACACGAG10500.062.8362167
CGGTGCC1650.061.517982
GGTGCAT4500.059.613853
Produced by FastQC (version 0.11.2)