FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_379_rr.351000000a9845.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_379_rr.351000000a9845.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1927709
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT38170.19800706434425527No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC33520.17388516627769027No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT28440.14753264107808803No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT27540.14286388661359156No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC25560.13259262679169936No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT23850.12372199330915609No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT23850.12372199330915609No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT23490.12185449152335752No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC22520.11682261171162246No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC22030.11428073428095215No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT21920.11371010873529148No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT21760.11288010794160322No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT20430.10598072634406958No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA19990.10369822416142686No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT19750.10245322297089447No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC19690.10214197267326137No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA19690.10214197267326137No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC19550.10141572197878415No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACG100.0070915855144.99852145
CCCCCTC100.0070915855144.99852145
GAGCAGA2500.0130.515581
GTAACAA25800.0123.0865254
GGTGCAT4650.0118.499563
CGGTGCA4750.0116.001822
GAAACAC9500.0109.136124
AAACACG10500.098.742215
TAGAAAC11500.095.82762
CACGCAC850.093.827443
TAACAAG35450.089.1715555
CGCACTT900.088.61485
CGGAGCG1150.088.262262
GTTACCG353.436051E-582.867041
GGAGCGT1250.081.2033843
AGCACGC900.080.565181
AACACGT900.080.5589146
GCACGCA1000.079.751262
ACAAGAG40100.078.648387
TAGTAAC42250.076.5337452
Produced by FastQC (version 0.11.2)