FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_377_rr.351000000a9838.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_377_rr.351000000a9838.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462473
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAACAACAGCTATGAGTGACTTGGCTAAGATTCACCCATGGGTCACTC20890.14284024388826325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT6000.077.332595
TAGTAAC12000.053.7721522
ACGCACT10000.052.9244884
AGCACGC10500.050.4060021
GTAGTAA13900.050.073171
GCACGCA11600.045.0011022
GTAACAA15350.041.090814
AACACGA6400.039.6480566
TATACCG1001.3918433E-436.249657
TACCGAT800.00203701936.249657
TAACAAG18350.035.1631475
ACACGAG7650.033.1696177
CACGCAC16100.032.8734783
AAGCGGT4650.031.1824957
ATACCGA950.004728225530.5260226
AAACACG10350.030.1204815
GAAGCGG7100.029.612396
AGAAGCG7700.029.188035
ACCGACT1000.00607565128.9997188
AGTAACA22900.027.8609453
Produced by FastQC (version 0.11.2)