FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_373_rr.351000000a982b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_373_rr.351000000a982b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3870167
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT50920.1315705497979803No Hit
GTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCATTATGACTTCC48020.12407733309699556No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT44280.11441366742055317No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAG15750.063.9843947
CACGAGC15750.063.5240758
CGCACTT7750.062.680925
ACGCACT8300.059.4009064
AAACACG19050.058.6121985
GCACGCA9300.053.015772
AACACGA19900.052.1009036
AGCACGC10050.049.060641
GTAACAA37500.048.3360334
ACGAGCC5250.048.332919
TAGTAAC38350.047.8337332
ACGAGCA13950.046.7737859
AGAAGCG12650.038.974515
CACGCAC13500.037.0590633
TAACAAG50850.035.5034565
GAAGCGG14150.034.8429376
AGTAACA54450.033.9563833
ACGAGCT5150.032.3783579
TAGAAAC44950.031.9385072
GAAACAC36000.031.6198224
Produced by FastQC (version 0.11.2)