FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_369_rr.351000000a981e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_369_rr.351000000a981e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770268
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC23730.1340475001525193No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT21800.12314519609460263No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC19760.11162151719400679No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG19650.11100014235132759No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC19260.10879708609091956No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT18400.10393906459360955No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT18000.10167951971113978No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA17870.10094516762433711No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG6450.066.3210455
CACGAGC5850.064.443938
ACACGAG6050.062.313557
CGCACTT753.7616155E-758.0028155
ACGAGCC2700.056.388449
CGGTGCG2250.051.5580525
AACACGA7650.050.2311976
GAGCAGA9000.049.1454351
TAGAAAC10350.048.335682
GTAACAA14600.048.1701434
GAAGCGG6100.046.3547066
GAAACAC9500.045.0285034
TAGTAAC15850.044.828672
AGAAGCG6400.044.181835
GCAGAAG10900.041.2405363
AACACGT1252.71948E-740.601976
GTGCGTT2850.035.613757
GGTGCGT3300.035.153226
AGCACGC1551.4643283E-632.7462961
ACGCACT1551.4652978E-632.7435234
Produced by FastQC (version 0.11.2)