FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_368_rr.351000000a9801.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_368_rr.351000000a9801.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541623
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT17680.11468432943722298No Hit
GTATATGCATACAATAGGATATTATTCATCCATAAAAGGGAATGAAGTAT17230.1117653278395561No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATTCTTTTAC16720.10845712602886698No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA16370.10618679145290386No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT15620.10132178879012574No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGA6900.057.79326
CGCACTT2400.054.3781475
ACACGAG7700.053.668467
AAACACG7750.053.3256725
ACGAGCC3400.049.0437779
CACGAGC8750.047.2282458
ACGCACT3150.043.7326934
AGCACGC3350.041.124451
GCGGTGC2550.039.8088071
GTAGTAA26200.038.7452161
GTAACAA25400.037.1084484
TAGTAAC25900.036.9519462
TAACAAG25650.036.181435
GCACGCA3850.035.7812962
AGCGAAT1051.854256E-434.523578
TATAACG1301.6547043E-533.4634742
AGAAGCG3300.032.9564555
AACAGCG1551.4650268E-632.743835
AGTAACA29950.032.4392783
GAAACAC13850.031.4097634
Produced by FastQC (version 0.11.2)