FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_366_rr.351000000a97f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_366_rr.351000000a97f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504321
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT21070.14006319130026104No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT4550.081.273025
AGCACGC5150.073.212241
ACGCACT5850.068.170174
GTAACAA23150.057.0043454
TAGTAAC23500.056.1553462
GTAGTAA24500.055.6390041
GCACGCA7200.053.3741462
AACACGA8550.052.5791056
ACGAGCG701.6965865E-551.784749
TAACAAG27900.047.559195
ACACGAG9500.047.31497
CACGAGC10000.044.224178
AGTAACA31050.042.734353
CGGTGCG1703.8198777E-1142.6519245
AAACACG11700.042.1415635
ACGAGCA5200.037.6435249
ACGAGCC3950.036.708179
ACAAGAG37000.035.8574337
CACGCAC11150.035.7664153
CAAGAGC33900.031.22368
Produced by FastQC (version 0.11.2)