FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_363_rr.351000000a97e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_363_rr.351000000a97e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411243
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCT753.7560312E-757.999329
AAACACG2600.055.7753565
CGAGCTT553.2150254E-452.7330675
ACACGAG2800.044.022697
CGCACTT855.332529E-542.6517455
AACACGA2750.042.186456
ATAGTAC1252.7113856E-740.6044583
GTACAGC907.475262E-540.2773061
CACGAGC2950.039.3263558
ACGCACT951.0271704E-438.1620864
TAGAAAC8700.032.503572
GAAACAC4950.032.225764
GTATTAC950.00472436530.5259551
ACGAGCC1204.0542104E-430.207989
AGCACGC1453.4857294E-529.999651
GTACCTT1703.0034607E-629.856226
ACACGTG1000.00606709629.0031877
CCTAGGG1504.3891967E-529.0031872
TAGTACC2252.8056093E-829.0031874
CACGCAC1255.1453186E-429.0031853
Produced by FastQC (version 0.11.2)