FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_361_rr.351000000a97d9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_361_rr.351000000a97d9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781956
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG50500.28339644749926485No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT38200.21437117414795878No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC36630.20556063112669448No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT28910.16223745142977716No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT27960.15690623113028604No Hit
GTATGCACTCTGTATCACCCAAGGGTTGTTACTAATACATTCTTCTATTC25420.14265223159269927No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC24750.13889231832884763No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT22530.1264340982605631No Hit
GTGCTTGTGATTCTAATTCACTTACATCATTGGCTCCCATTATGACCTCC22440.12592903528482186No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT22310.1251994998754178No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG21770.1221691220209702No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT21200.11897038984127555No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA21130.11857756308236568No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC21080.11829697254028719No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG19430.10903748465169735No Hit
AGACAATATAGACCCAAAAGGAGCAATAGAAAGAAATCTAGCAAGGATGT18440.10348179191854345No Hit
TCCTTGTATCGATGATTCTTGTTCAACAATTGCTTCCATTTGCTGCATTG18250.10241554785864523No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT17830.10005858730518598No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT17820.10000246919677029No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG6750.078.40955
GCACGCA755.638867E-1177.33542
AACACGA6800.074.6343466
TAGAGCG300.001935786672.501934
ACACGAG7200.070.487997
ACGAGCC3150.064.444369
AGCACGC902.8194336E-1064.444361
CACGAGC8100.063.551088
GAAACAC8750.060.4873284
CGGTGCA1900.057.2383732
CACGCAC1101.6771082E-952.728683
CGCACTT1101.6771082E-952.728685
GCGGAGC3200.049.843681
AGAAGCG5600.046.6083875
AGCGTTT1900.045.79075
GAGCGTT1751.8189894E-1245.5726474
AAGCGGT4600.044.1316157
TATAGAG13000.041.2703325
ACGAGCA7250.039.9999479
GTAACAA28900.039.6377374
Produced by FastQC (version 0.11.2)