FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_348_rr.351000000a97cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_348_rr.351000000a97cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676303
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT20290.12104016994540963No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT19680.11740120968583842No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC32800.0103.895642
GTAGTAA33000.0103.708491
CGCACTT10450.092.974075
GTAACAA37800.091.111894
AGCACGC11800.078.038331
TAACAAG43800.077.803125
AGTAACA45350.076.10323
ACGCACT14850.066.890954
GCACGCA14700.063.6276172
AAGAGCG10150.061.4280669
ACGAGCG2700.059.073599
ACAAGAG62600.054.895727
CAAGAGC62850.052.6009988
AACAAGA69550.049.310266
AAACACG18700.046.5278435
TCTCGCT7250.046.003742
CACGCAC22250.043.34063
CTCGCTC7650.042.6505283
TCGCTCT8050.040.5312424
AACACGA17500.039.774666
Produced by FastQC (version 0.11.2)