FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_347_rr.351000000a97bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_347_rr.351000000a97bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686405
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT34570.20499227646976853No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT29040.1722006279630338No Hit
AAGCAGAGCAGACCACAAACAGAATAAACAAAAGGTAAATTGTTGGACTT23560.1397054681408084No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT22060.13081080760552774No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA21630.12826100491874726No Hit
ACAACAGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGA21420.12701575244380797No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG21210.1257704999688687No Hit
TGTCCTCCAGATTCACCTACATTGTGGCAAATAGCAGGAGCCCTCTTTTT20840.12357648370349945No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG19450.11533409827413937No Hit
GAGTAACACCTGGGAAAGCTTCACCAGAGGGTCCTTTTCCACCACAGCAA19010.11272499785045705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT18500.0112.080695
TAGTAAC34250.0111.134292
GTAGTAA35400.0108.138421
AGCACGC18750.0107.109471
GTAACAA40250.096.1860054
GCACGCA21000.095.633462
TAACAAG44300.086.246885
AAGAGCG10900.079.816249
ACGCACT29100.071.752334
AGTAACA56400.068.2597663
CACGCAC31250.065.425713
AACACGA28050.060.9998131
CGGTGCA8100.057.2854462
CGAGCGG2200.056.0225335
ACAAGAG69400.054.949377
ACGAGCG3450.054.637494
GCGGTGC13550.051.9017261
CGAGCTA6100.051.106385
AACAAGA74800.050.8855136
GTAGAAA31450.050.4862171
Produced by FastQC (version 0.11.2)