FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_346_rr.351000000a97a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_346_rr.351000000a97a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126876
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA24870.11693206374043433No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT5850.076.838965
ACGCACT8350.058.174684
GTAGTAA22850.052.6692771
TAGTAAC22600.052.6115532
AGCACGC9800.048.826241
GCACGCA10800.044.306342
GTAACAA27000.044.0378154
AAGAGCG10000.042.0497559
TAACAAG30300.038.763065
AACACGA10650.037.441986
ACGAGCG1553.473724E-837.4191369
AGTAACA32350.036.7548983
ACACGAG12400.032.742517
AAACACG14350.031.8298285
CACGAGC13300.029.9817338
ATTAGTA17500.029.4141141
CACGCAC18100.027.6386093
ATACCGA1658.421937E-526.3641036
GTAGAAA37350.025.428231
ACAAGAG48500.024.0674367
Produced by FastQC (version 0.11.2)