FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_344_rr.351000000a9796.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_344_rr.351000000a9796.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560641
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT44750.2867411531543769No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC22380.14340261469485938No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT21710.13910950692696142No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG19050.1220652283260532No Hit
ATATAGACCCAAAAGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC18370.1177080443228135No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT18290.11719543444007943No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC17800.11405569890833317No Hit
AGACAATATAGACCCAAAAGGAGCAATAGAAAGAAATCTAGCAAGGATGT17050.10924998125770116No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA16630.10655877937334723No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT16310.10450833984241091No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT3700.080.3373645
AGCACGC4050.076.974851
GCACGCA4400.070.851852
AAACACG9050.069.695725
ACGCACT4950.065.90874
AACACGA8800.060.9655466
CGGTGCA2400.054.3746762
CACGCAC6000.051.958023
GAAACAC12650.051.00764
GTAACAA27900.050.4118844
ACACGAG10500.050.404467
TAACAAG29200.048.167525
TAGTAAC31600.046.3446582
AGCGTTT1109.964606E-846.136095
GTAGTAA32900.046.0559541
CACGAGC11500.046.021468
AGAAGCG4850.041.855425
AAGCGGT3750.040.599767
ACGAGCC4300.038.778849
ACAAGAG38350.037.998477
Produced by FastQC (version 0.11.2)