FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_340_rr.351000000a9789.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_340_rr.351000000a9789.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412502
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA4600.066.1974642
CGCACTT4050.064.4462055
AAACACG7750.051.453025
ACGCACT6150.049.513554
AACACGA7350.049.321086
AGCACGC6250.048.7213331
CACGAGC7650.047.3869138
ACACGAG7800.046.475637
CACGCAC7700.041.4297033
TAGTAAC15100.039.3719372
GTAGTAA18250.036.5489431
CGGTGCG800.002036626236.2509887
ACGAGCG850.00274247534.1173749
GAAGCGG6250.033.640921
TCAACGG900.003628247432.2231035
AAGCGGT3450.031.5225987
ACGAGCC4950.029.2926949
ACGAGCT3750.028.9997679
GTAACAA20250.028.6427574
AGAAGCG5650.028.230865
Produced by FastQC (version 0.11.2)