FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_338_rr.351000000a977c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_338_rr.351000000a977c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931819
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA23460.12143994856661001No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG23320.1207152429911912No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT21400.11077642367116174No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA21120.10932701252032412No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC20640.10684230769031675No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG19780.10239054486988687No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGA5950.075.549496
AAACACG6350.074.215825
CACAGCG300.001935683472.503143
GAGCAGA7000.069.399461
ACACGAG6600.067.007027
CACGAGC6750.065.5179758
ACGAGCC2550.065.391619
GAAACAC7450.063.257784
CGGTGAG400.006051936754.374546145
AGAAGCG5200.050.1944855
ACGCACT657.3367206E-444.6173174
GAAGCGG5750.044.132356
GTGTAGG1002.7336828E-643.504141
AGCACGC855.3403513E-542.6511151
CGGAGCC855.341983E-542.648913
GTAACAA22950.042.017074
GCACGCA700.001056390941.4325142
CGGTGCA1755.2750693E-1141.4325142
ACACGTG700.001056929241.4282267
CAGAAGC11400.040.7035184