FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_337_rr.351000000a9739.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_337_rr.351000000a9739.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2442615
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA47150.0104.8794861
TAGTAAC49400.099.949662
CGCACTT13950.096.148155
GTAACAA55050.089.687774
TAACAAG63500.077.86715
AGTAACA64100.076.9136053
ACGCACT19150.071.175824
AGCACGC18700.068.2431261
GCACGCA19450.065.607612
ACAAGAG94400.052.4556277
CAAGAGC94000.050.7506338
AAGAGCG12750.050.6074339
CACGCAC26350.049.2520943
CGAGCGA1800.048.3339355
AACAAGA102550.048.2868046
AACACGA30900.045.053711
AAACACG27750.044.15375
CGGTGCA8550.044.0959172
ACGAGCG3300.043.939049
GTAGAAA48100.040.0981561
Produced by FastQC (version 0.11.2)