FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_333_rr.351000000a972c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_333_rr.351000000a972c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979722
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC69730.35222117044716383No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA50390.25453068663175943No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT38360.19376457906716196No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA34660.17507508629999566No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT34380.17366074630680467No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA32600.16466958492151929No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT29080.14688931072140432No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC28580.14436370359070616No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT26880.13577663934633247No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG25590.12926057294913124No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC25150.1270380386741169No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATCCTTTTAC25020.12638138082013536No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA24610.12431038297296287No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG23920.12082504513259942No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT23880.12062299656214358No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG23870.12057248441952961No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT23840.12042094799168772No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA23100.11668304943825447No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT23020.11627895229734275No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG22200.1121369566029978No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA21300.10759086376774113No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACTGGTGTTCCCCC20070.10137787022622367No Hit
ATCCAATGATGACCAATAACCCCATATACATCTTCGGAGCTTATGTGTAC19990.10097377308531197No Hit
ATATAAATCCTTACTTTCTCTTCATAGATGTACCCATACAGGCAGCAATT19980.10092326094269802No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA19890.10046865165917235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA1500.096.6666262
AGCACGC1650.087.88321
CGCACTT1650.087.878765
AAACACG10050.087.288535
GAAACAC10650.083.732374
CGGTGCA2750.081.727252
AACACGA11000.073.818166
GCCTACT406.6631415E-572.503641
CACAGCG502.3070806E-672.499983
ACGAGCG300.001936026372.499989
ACGCACT2300.066.1956254
CACGCAC2300.066.1956253
GTAACAA28700.065.932044
ACACGAG12500.064.959987
CACGAGC13900.057.8956688
GAGCAGA9200.055.1658131
TAGTAAC36700.052.3501172
ACGAGCC7350.052.2788939
GGTGCAT4500.049.9444273
TAACAAG37750.049.7417075
Produced by FastQC (version 0.11.2)