FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_328_rr.351000000a9702.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_328_rr.351000000a9702.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092431
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG48670.2326002625654084No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC37180.17768805757513628No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT34160.16325508463600474No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT29200.13955059927902042No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT26170.12506983503876593No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT25940.123970635112938No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC24690.117996722472569No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT22340.10676576670867523No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA22330.10671797540755226No Hit
GCTTTGGTCATGTCATATCCTTGTATCGATGATTCTTGTTCAACAATTGC22200.1060966884929539No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA22110.1056665667828473No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT21930.10480632336263417No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT21650.10346816693119151No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG21360.10208221919862591No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATCCTTTTAC21190.10126976707953571No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG7000.082.860765
AACACGA7200.073.5101556
ACACGAG8200.064.543967
CGCACTT1350.064.4472665
CACGAGC8950.059.135258
GAAACAC10400.056.468824
CGGTGCA4250.056.2979242
AGAAGCG5050.054.556845
CACGCAC1700.051.179933
AGCACGC1800.048.3377531
GCACGCA1800.048.33662
ACGCACT1800.048.3354454
ACGAGCC2800.046.606959
GCGGAGC5000.044.954111
GCGGTGC7050.044.2239041
AAGCGGT4600.044.1313067
GAAGCGG6700.041.1211976
ACGAGCA9550.040.9945959
ACGAGCT1157.1466475E-637.825939
GGTGCAT6700.036.793533
Produced by FastQC (version 0.11.2)