FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_327_rr.351000000a96e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_327_rr.351000000a96e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2288558
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT47180.20615601614641185No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT38690.1690584201929774No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT33100.14463255901751235No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT29440.1286399558149717No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC28510.12457626155858842No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA27790.1214301756826788No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA27680.12094952367385926No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT27550.1203814803907089No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG27420.11981343710755857No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT25880.11308430898408518No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT25410.11103061403731082No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG24390.10657365904643884No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC24110.10535018120580732No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC23130.101068008763597No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA23130.101068008763597No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC22900.10006300910879251No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA4400.0117.005631
CAGGATT1950.0111.54985
CACAGGA2200.0105.465253
GTAACAA18500.090.144294
GGTGCAT3950.089.945843
CGGTGCA4050.087.724962
GAAACAC7850.086.8241044
AAACACG8000.085.196155
CGGAGCA2950.083.567812
TAGAAAC10300.073.211322
AACACGA8850.072.097726
TAGTAGA2250.070.896093
ACACGAG9400.067.873317
GCGGAGC4750.067.1676561
CACGTGC1450.065.009441
ACGTGCA902.8194336E-1064.4512
TACACCC350.003565655862.149174
AGAAGCG5150.061.948045
TAGTCTC502.0093509E-458.008431
AACACGT753.7607242E-758.0058946
Produced by FastQC (version 0.11.2)