FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_325_rr.351000000a96cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_325_rr.351000000a96cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634681
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT5400.067.131135
TAGTAAC16300.060.4921532
AGCACGC7350.053.2664951
GTAGTAA19500.052.052451
CACGCAC8400.048.3344153
ACGAGCG901.3241715E-648.3329359
ACGCACT8000.048.0323264
CACGAGC7750.047.7092828
TAACAAG21600.044.9778565
AACACGA8400.044.0188456
GCACGCA9000.043.5009772
ACACGAG8850.041.779327
GTAACAA23850.040.430674
AAACACG11700.037.799995
AGTAACA25550.037.740573
CCATACG2650.035.5668335
ACGAGCT3100.035.0803579
TACTCGC1153.1625564E-431.5224466
GCGTTGC1902.0781044E-730.526064145
GTAGAAA28500.028.7462561
Produced by FastQC (version 0.11.2)