FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_323_rr.351000000a96a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_323_rr.351000000a96a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800415
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT6000.088.210395
GTAGTAA24250.082.5196841
TAGTAAC24300.081.4525152
GTAACAA31950.062.8574944
ACGCACT9150.059.4276164
AGTAACA35550.056.696123
AAGAGCG8600.048.8951389
TAACAAG40950.048.8656125
AGCACGC11150.046.168281
GCACGCA11250.045.7566262
AAACACG16150.041.3012775
ACAAGAG51550.038.8175937
AACACGA17000.038.8108061
CGGTGCG750.001483531938.667575
ACGAGCG1553.4731784E-837.419199
AAGCGGA4150.036.6876072
CAAGAGC51850.036.355728
CACGCAC14700.036.0042423
GAAGCGG9100.035.853481
ATTAGTA15400.033.8978351
Produced by FastQC (version 0.11.2)