FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_322_rr.351000000a968a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_322_rr.351000000a968a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023227
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT24500.12109367856399703No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT23490.11610165344768532No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT9800.0105.0584265
GTAGTAA38900.099.349951
TAGTAAC39500.097.2853242
GTAACAA46350.083.533394
ACGCACT13400.077.915934
AGTAACA51600.075.0343553
TAACAAG52350.074.513365
AGCACGC15050.066.968161
GCACGCA15700.063.7306022
AAGAGCG11800.061.440459
ACAAGAG71650.053.9334377
CACGCAC19200.052.113043
CAAGAGC71050.051.327618
AAACACG21400.050.4825255
AACAAGA84300.046.358546
ACGAGCG2350.046.279854
AACACGA23850.042.866771
TCGCTCT9050.041.6603934
TCTCGCT9350.040.3236962
GTAGAAA40700.039.728351
Produced by FastQC (version 0.11.2)