FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_320_rr.351000000a9660.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_320_rr.351000000a9660.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497405
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT6050.097.0695
GTAACAA28450.089.958744
TAGTAAC31650.081.092462
GCACGCA7300.077.468062
ACGCACT8200.073.386334
TAACAAG36050.071.597135
AGCACGC7900.071.586791
GTAGTAA37550.069.1255341
AGTAACA42050.061.5535553
CGGTGCA4800.060.418462
ACAAGAG45300.056.4972657
AAACACG14900.052.5518955
GAAACAC15700.050.797694
CACGCAC11850.049.5584343
CATACGG450.00963766348.3363841
CGAGCGG604.9451506E-448.334775
GCGGTGC9600.046.825871
AACACGA15750.045.5743031
CTCGCTC4100.044.208633
ACGAGCT5800.043.75134
Produced by FastQC (version 0.11.2)