FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_318_rr.351000000a9647.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_318_rr.351000000a9647.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215823
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT12850.082.93765
GTAGTAA31500.074.34451
ACGCACT14950.074.19724
AGCACGC16200.067.1325451
GTAACAA35250.065.815494
TAGTAAC36150.064.1783752
GCACGCA18600.058.0791662
AGTAACA43450.053.8964043
TACGTCA553.219885E-452.727185
TAACAAG48300.048.3332525
AACACGA15450.045.9869776
CACGCAC25100.043.6164473
AAGAGCG11500.041.6086279
ACGTGCG700.001056928141.42859
AAACACG20250.040.8147475
ACGAGCG2150.040.4650469
CTGCGCG1105.2672676E-639.545387145
ACAAGAG60900.038.2142227
CGGTGCG951.0291693E-438.158692
ACACGAG20050.035.4363487
Produced by FastQC (version 0.11.2)