FastQCFastQC Report
Thu 23 Nov 2017
HW23FBCXY_l01n01_s1_316_rr.351000000a962d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW23FBCXY_l01n01_s1_316_rr.351000000a962d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2574238
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAA56580.21979319705481776No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT42870.16653471823506605No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC36880.14326569648960197No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA36590.1421391495269668No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG33520.13021329030182913No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT33190.1289313575512443No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA29930.11626741583334564No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT29050.11284892849845274No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT28930.11238277113460372No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT28100.10915851603464793No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT27980.10869235867079889No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT27810.10803196907201278No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT27310.10608964672264182No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC27270.10593426093469212No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT26880.10441924950218277No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT26670.10360347411544697No Hit
ATTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACA26300.10216615557691246No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA100.007091287145.00152
GTAACAA29250.0116.00124
GGCTCGG203.866896E-4108.749011
GAAACAC14800.0107.281514
CGGTGCA5100.0105.197172
GAGCAGA3700.0103.850411
GGTGCAT5250.0102.191533
GCACGCA501.9463187E-10101.501052
AAACACG15800.0100.4915545
TAGAAAC16400.0100.351652
AGCACGC554.1654857E-1092.271881
CGCACTT850.085.294995
AACACGA18550.081.294646
ACGCACT900.080.556384
TAGTAAC42750.080.3867952
CACGCAC651.5661499E-978.077733
CGGAGCA4950.077.627062
GCGGAGC7300.075.478771
ACAAGAG46300.073.28377
TAGTAGA2600.072.500753
Produced by FastQC (version 0.11.2)